Advantages of Biological Buffer Tris

In biochemical research, biological buffers are often used, and there are as many as 20-30 varieties of biological buffers. Biological buffers are widely used in the in vitro diagnostic industry and biochemical experiments. What are the advantages of Tris in biological buffers?

• Tris has high solubility in water and is inert to the reaction of many enzymes.
• Tris has a high buffering capacity. It is generally used to stabilize the pH of the reaction system. It has a strong buffering capacity between pH 7.5-9.0.
• Tris is very alkaline, so this buffer system can be used to configure a wide range of pH buffers ranging from acidic to alkaline.
• Tris has little interference to the biochemical process and will not react with calcium, magnesium ions and heavy metal ions.
• Tris buffer is widely used, and plays an important role in the fields of biochemistry, cosmetics, in vitro diagnostics, molecular biology and coatings.

When using Tris buffer, it is also necessary to note that the pH value of Tris is greatly affected by temperature and concentration, so the use environment should be considered during the preparation process. Tris will easily absorb CO2 in the air, and the prepared buffer should be tightly sealed to avoid loss. In addition, Tris will react with various molecules to a certain extent, including RNase inhibitors, aldehydes, enzymes, DNA, and common metals. It can also act with heavy metals, but it also has an inhibitory effect on some systems.