Prescription

Tris Base are the core raw material for nucleic acid extraction after strict screening and quality control. Buffer solution is a kind of solution that can resist the influence of a small amount of strong acids and bases and maintain the pH value of the system unchanged. It is very important to maintain pH 4.0-p11.0 in the process of nucleic acid extraction. Nucleic acid is easy to hydrolyze in an acidic solution and stable in a neutral or weak alkaline solution.

Application

• Tris is widely used in acute metabolic and respiratory acidemia, is an alkaline buffer, and it has a good buffering effect on metabolic acidosis and enzyme activity.
• Tris is commonly used as a biological buffer, often prepared to pH 6.8,7.4,8.0,8.8. Its structural formula and pH value vary greatly with temperature. Generally, the pH decreases by 0.03 for each degree of temperature increase.
• Tris is widely used in the preparation of buffers in biochemistry and molecular biology experiments. For example, TAE and TBE buffers commonly used in biochemistry experiments (for nucleic acid dissolution) require Tris; since it contains amino groups, condensation reactions with aldehydes can occur.