Uses of Tris:

1. Chemical raw material: Tris is widely used as an important chemical raw material for flame retardant materials, coatings and amino resins, etc.
2. Reaction medium: As an excellent reaction medium, Tris is often used in organic synthesis reactions, which can play the role of catalyst.
3. Resin cross-linking agent: Tris(hydroxymethyl)aminomethane can also be used in resin cross-linking reaction, which can improve the hardness and heat resistance of resin.
4. Biological buffering agent: In the field of medicine, Tris is used as a biological buffering agent, which is widely used in cell culture, DNA/RNA extraction, PCR amplification, etc.

Configuration method of Tris solution:

1. Weigh the required amount of Tris powder and add it to sterile high-purity water.
2. Use a magnetic stirrer to dissolve the Tris powder until it is fully homogeneous.
3. Calculation of concentration: Generally, the concentration of trimethylolaminomethane is 1 mol/L, and the concentration can be adjusted appropriately according to the need.
4. Measure the PH value of the solution with a PH meter, which should generally be controlled between 7.0 and 8.5.
5. Preparation of buffer solution: standardize the solution to the required volume, usually used as a buffer solution with a concentration of 50-200 mmol/L.
6. Sterilization: The trimethylolaminomethane solution is loaded into sterile bottles and sterilized according to a certain process.