Tris (hydroxymethyl) Aminomethane
Tris name: Tris; Tromethamine; Tromethamine; 2-amino -2-(hydroxymethyl)-1, 3-propanediol. Tris is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate, benzene, insoluble in ether, carbon tetrachloride, copper, aluminum corrosion, irritant chemicals.
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Application
Tris buffer is not only widely used as a solvent for nucleic acids and proteins but also has many important applications.
1. Tris is used for protein crystal growth under different pH conditions.
2. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in c. elegans.
3. Tris is also one of the main components of protein electrophoresis buffer.
4. Tris is used as an intermediate in the preparation of surfactants, vulcanization promoters and some drugs.
5. Tris is also used as a titration standard.
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| Item | Specifications | ||||||||||||||||||||||||||||||
| Appearance | White crystal powder | ||||||||||||||||||||||||||||||
| Content | 99.5%-101.0% | ||||||||||||||||||||||||||||||
| Moisture(by karl fischer) | ≤0.3% | ||||||||||||||||||||||||||||||
| Insolubles | ≤0.005% | ||||||||||||||||||||||||||||||
| PH(5%) | 10.0-11.5 | ||||||||||||||||||||||||||||||
| UV Absorbanc(290nm,40%) | ≤0.2 | ||||||||||||||||||||||||||||||
| UV Absorbance(400nm 40%) | ≤0.2 | ||||||||||||||||||||||||||||||
| Heavy metals(as pb) | ≤5ppm | ||||||||||||||||||||||||||||||
| Fe | ≤5ppm | ||||||||||||||||||||||||||||||
| Cl | ≤3ppm | ||||||||||||||||||||||||||||||
| Solubility (1.0M solution in water) | Immediately soluble yielding a clear haze-free solution free of particulates | ||||||||||||||||||||||||||||||
| Residue on ignition | ≤0.05% | ||||||||||||||||||||||||||||||
| Melting point(ºC) | 168.0-172.0 | ||||||||||||||||||||||||||||||
| Conclusion | The results conforms with Enterprise standards
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